Pre-mRNA splicing is performed by the sequential function of different spliceosome complexes. Spliceosome assembly depends on the presence — from the 5′ end to the 3′ end of introns — of conserved 5′ ...
The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E ...
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